All steps for stimulation of ovaries and egg retrieval procedure are the same as IVF. After the granulosa cells have been stripped away from the oocyte with enzymatic action, the oocyte is held in place by a holding pipette. A single selected sperm is first immobilized and then drawn into the pipette carrying sperm pierces the oocyte wall and sperm injected directly into the ooplasm. The morning after insemination, the eggs are examined to determine whether fertilization has occurred.